Tailorable Bioinks for Bioprinting of Artificial Skin Substrates

Problem Statement

Every year, patients with extensive burns and chronical skin wounds face substantial challenges including physical pain, physiological distress and economic burdens. Although significant progression has been made in producing skin substitutes for wound treatment, a skin substitute that can promote tissue regeneration in the way comparable to the autologous skin graft is still not available yet.

Objective

This project aims to develop a bioprinting technology and formulation of novel bioink for the production of artificial skin substrates.

Steps in the production of proposed bioprinted skin substitutes
Steps in the production of proposed bioprinted skin substitutes

Key Benefits/Outcomes

  • Developed a new bioink for bio-printing of skin construct, and successfully utilized bioprinting to prepare 2D scaffolds for cell culture.
  • Successfully constructed the artificial skin model that could support the growth of fibroblasts and keratinocyte co-cultures, and could promote stratification of keratinocytes.
  • Novel bioink combines synthetic polymers and bioactive proteins to simultaneously exploit the advantages of both systems. Benefits includes:
    • Compatible with current bioprinters
    • Has good printability & can produce scaffolds of multiple layer
    • Tunable mechanical properties (by using appropriate MW of PEGDA)
    • Good bioactivity to promote attachment and growth of cells
  • Two Technology Disclosures have been filed by NTU on the bioink and bioprinting techniques.

Collaborator

Contacts

Mr. Marc Lee marc.lee@ntuitive.sg